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科研成果

一、八项国家标准

1. 《马铃薯脱毒试管苗繁育技术规程》(GB/T 29375-2012

2. 《马铃薯脱毒原原种繁育技术规程》(GB/T 29376-2012

3. 《马铃薯脱毒种薯生产技术规程》(GB/T 29378-2012

4. 《马铃薯脱毒种薯级别与检验规程》(GB/T 29377-2012

5. 《马铃薯脱毒种薯贮藏、运输技术规程》(GB/T 29379-2012

6. 《马铃薯商品薯生产技术规程》(GB/T 31753-2015)

7. 《马铃薯商品薯分级与检验规程》(GB/T 31784-2015)

8. 《马铃薯商品薯质量追溯体系的建立与实施规程》(GB/T 31575-2015)

二、发表论文

1. Miru Du, Xiangyu Ren, Qinghua Sun, Yi Wang, Ruofang Zhang. (2012) Characterization of Fusarium spp. Causing Potato Dry Rot in China and Susceptibility Evaluation of Chinese Potato Germplasm to the Pathogen. Potato Research. 55:175–184

2. Wu, L., Bhaskar, P.B., Busse, J.S., Zhang, R.F., Bethke, P.C., and Jiang, J. (2011) Developing cold-chipping potato varieties by silencing the vacuolar invertase gene. Crop Sci. 51: 981-990.

3. Xie, KY; Wang, XX; Zhang, RF; Gong, XF; Zhang, SB; Mares, V; Gavilan, C; Posadas, A; Quiroz, R. 2011. Partial root-zone drying irrigation and water utilization efficiency by the potato crop in semi-arid regions in China. Scientia Horticulturae. 134(1):20–25

4. Zhang, R; Van Eck, HJ; Carlson, JE; Haynes, KG; Christ, BJ. 2004. Quantitative trait loci for partial resistance to early blight and foliage maturity in a full-sib diploid potato family. Phytopathology. 94(8):867-877

5. Zhang, R; Haynes, KG; Christ, BJ. 2003. Inheritance of resistance to early blight disease in tetraploid x diploid crosses of potato. Amer J Potato Res. 80(1):76-80

6. Zhang, R; Carlson, J E; Van Eck, HJ; Haynes, KG; Christ, BJ. 2003.Genetic maps for a full-sib diploid potato family using fluorescence-based, semi-automated AFLP and SSR markers. Genome. 46(4):612-626

7. Wu, L.,and Jiang, J. Suppression ofthe vacuolar invertase gene prevents cold-induced sweetening in potato. Plant Physiology, 2010, 154(2):939-948.

8. Du M , Luo M , Zhang R , et al. Imprinting in rice: the role of DNA and histone methylation in modulating parent‐of‐origin specific expression and determining transcript start sites[J]. The Plant Journal, 2014, 79(2).

9. Xuanyuan G , Lu C , Zhang R , et al. Overexpression of StNF-YB3.1 reduces photosynthetic capacity and tuber production, and promotes ABA-mediated stomatal closure in potato (Solanum tuberosum L.)[J]. Plant Science, 2017, 261:50-59.

10. Wei C , Liu J , Maina A N , et al. Developing a bacteriophage cocktail for biocontrol of potato bacterial wilt[J]. Virol Sin, 2017, 32(006):476-484.

11. Bao S , Zhang Z , Lian Q , et al. Evolution and expression of genes encoding TCP transcription factors in Solanum tuberosum reveal the involvement of StTCP23 in plant defence[J]. BMC Genetics, 2019, 20.

12. Bao S, Owens RA, Sun Q, Song H, Liu Y, Eamens AL, Feng H, Tian H, Wang MB, Zhang R. Silencing of transcription factor encoding gene StTCP23 by small RNAs derived from the virulence modulating region of potato spindle tuber viroid is associated with symptom development in potato. PLoS Pathog. 2019 Dec 2;15(12):e1008110. doi: 10.1371/journal.ppat.1008110. PMID: 31790500; PMCID: PMC6907872.

13. Du, M., and Qi, J. Developing a new model system for potato genetics by androgenesis. Journal of Integrative Plant Biology, 2021, 63(4):6

14. Cao Y , Sun Q , Feng Z , et al. First Report of Pectobacterium parmentieri Causing Blackleg on Potato in Inner Mongolia, China[J]. Plant Disease, 2021.14.

15.Handique U , Zhang R , Zhang Z , et al. First Report of Streptomyces stelliscabiei Causing Potato Common Scab in Guizhou Province, China[J]. Plant Disease, 2021.

16. 中国马铃薯主产区晚疫病群体遗传结构研究 作者:轩辕国超,王彦凤,郭海英,毛锐涛,王姝梅,张若芳.

17. 沙地喷灌土壤基质势调控对土壤水分分布和马铃薯产量的影响. 作者:冯志文等.灌溉排水学报(CSCD),2019.

18. 滴灌施肥条件下减量施肥对马铃薯田土壤养分积累及产量的影响.作者:冯志文等.节水灌溉,2019.

19.贮藏期马铃薯干腐病防治药剂筛选及其安全性评价 作者:孙清华等.

20. 10种杀菌剂对马铃薯黑胫病主要致病菌的室内毒力 作者:冯志文等.

三、科研成就

1. 在国际上首次对马铃薯二倍体原始栽培品种S.phureja×S.stenotomum的半同胞家系群体的抗病性进行了系统的实验和遗传分析研究,证实该群体具有较高水平的早疫和晚疫病数量遗传抗性,首次建立了S.phureja×S.stenotomum半同胞家系群体的基因组图谱,对研究定位该群体高淀粉、抗多种病害、抗霜冻和其它抗逆优良性状的分子遗传机制和育种具有重要意义。

2. 通过RNAi的方法构建了可高效表达一种、两种和三种病毒dsRNA的载体,从而沉默特定基因,为马铃薯抗病毒提供深入的理论研究,为有效防治病毒病提供新的手段。在此基础上构建沉默马铃薯GBSS基因的载体,获得高支链淀粉转基因马铃薯,并将马铃薯抗病与品质特性相结合,获得既抗病毒、又含高支链淀粉的转基因马铃薯。目前,这一新品种/品系正在进行抗性实验。

3. 利用现代分子生物学技术,建立起多重Real-Time PCR检测马铃薯病毒的方法,目前能够检测马铃薯X病毒、Y病毒、卷叶病毒、M病毒、S病毒和马铃薯块茎纺锤类病毒PSTVd(国家检疫病毒病),2个细菌病害和3个真菌病害。此技术具有操作简单、快速便捷、重复性好、污染率低等优点,且灵敏度比目前国际通用监测方法提高了100-1000倍。在马铃薯种薯质量监测管理体系和种薯标准化繁育技术体系体系中,发挥着重要的作用。


内蒙古自治区马铃薯工程技术研究中心
内蒙古大学马铃薯工程技术研究中心

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